anti pde10a (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti pde10a
Anti Pde10a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pde10a/PDE10A+Antibody/10__1515_slash_nipt___2025___0009-48-24-29
Average 93 stars, based on 16 article reviews
Anti Pde10a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pde10a/PDE10A+Antibody/10__1515_slash_nipt___2025___0009-48-24-29
Average 93 stars, based on 16 article reviews
anti pde10a - by Bioz Stars,
2026-09
93/100 stars
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Blocking Assay:Article Title: Phosphodiesterase 10A Is Tethered to a Synaptic Signaling Complex in Striatum Article Snippet: Western Blot Protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes (Protran BA-85, Schleicher & Schuell) using standard procedures. .. After blocking (Roti-Block; Carl Roth), proteins were detected using the following antibodies: Article Title: Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells Article Snippet: After SDS PAGE, the gel was incubated with CAPS buffer (50 mM CAPS, 10% methanol, and 1 mM mercaptopropionic acid, pH 10.0) for 10 min. Proteins were semidry blotted for 1 h at 1 mA/cm 2 at room temperature to a stabilized nitrocellulose membrane (Optitran BA-S, Schleicher and Schuell, Dassel, Germany) using a Mini Protean unit (BioRad, München, Germany). .. Membranes were blocked with 5% nonfat milk powder or 5% BSA in TTBS buffer (0.1 M Tris, 150 mM NaCl, and 0.1% Tween-20), respectively, and incubated with Incubation:Article Title: Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus Article Snippet: .. Proteins were transferred to Immobilon-P membranes (EMD Millipore) and membranes were incubated with the following primary antibodies: β-actin (AC-15, 1:10,000, Sigma–Aldrich), PDE1A (sc-50480, 1:4,000, Santa Cruz Biotechnology), PDE1B (ab14600, 1:500, Abcam; ), PDE1C (sc-67323, 1:500, Santa Cruz Biotechnology; ), PDE3A (sc-20792, 1:250, Santa Cruz Biotechnology; ), phospho-PDE4A (GTX14610, 1:2,000, GeneTex), PDE4A5 (ab42094, 1:2,000, Abcam; ), PDE4A8 (GTX14606, 1:1,000, GeneTex), PDE4B (sc-25812, 1:500, Santa Cruz Biotechnology; ), PDE4D (sc-25814, 1:500, Santa Cruz Biotechnology; ), phospho-PDE4D (ab59212, 1:1,000, Abcam), PDE8A (sc-30059, 1:500, Santa Cruz Biotechnology; ), PDE8B (sc-17234, 1:500, Santa Cruz Biotechnology; ), and Article Title: Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells Article Snippet: After SDS PAGE, the gel was incubated with CAPS buffer (50 mM CAPS, 10% methanol, and 1 mM mercaptopropionic acid, pH 10.0) for 10 min. Proteins were semidry blotted for 1 h at 1 mA/cm 2 at room temperature to a stabilized nitrocellulose membrane (Optitran BA-S, Schleicher and Schuell, Dassel, Germany) using a Mini Protean unit (BioRad, München, Germany). .. Membranes were blocked with 5% nonfat milk powder or 5% BSA in TTBS buffer (0.1 M Tris, 150 mM NaCl, and 0.1% Tween-20), respectively, and incubated with Western Blot:Article Title: Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice. Article Snippet: Samples were fractionated by SDSPAGE and transferred to nitrocellulose (GE Amersham, 10600002). .. Immunoblots were probed with the following primary antibodies and visualized by ECL (Thermo Fisher, 32106): NLRP3 (Adipogen, AG-20B-0014; 1:1000), CASP1/caspase-1 (Adipogen, Ag-20B-0042; 1:1000), IL1B/IL1β (R&D, AF401; 1:1000), SNCA/ α-synuclein (Abcam, ab1903; 1:1000), Ubiquitin Proteomics:Article Title: Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice. Article Snippet: Samples were fractionated by SDSPAGE and transferred to nitrocellulose (GE Amersham, 10600002). .. Immunoblots were probed with the following primary antibodies and visualized by ECL (Thermo Fisher, 32106): NLRP3 (Adipogen, AG-20B-0014; 1:1000), CASP1/caspase-1 (Adipogen, Ag-20B-0042; 1:1000), IL1B/IL1β (R&D, AF401; 1:1000), SNCA/ α-synuclein (Abcam, ab1903; 1:1000), |